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DNA Tm Calculator can quickly calculate the Tm of the DNA/PCR primer to help designing primers for PCR/RT-PCR and other DNA amplification techniques. The calculations are based on Breslauer's nearest neighbor analysis of deltaH and deltaS.
App Features:
* Tm is calculated as the bases are entered.
* Primer concentration is factored in to Tm Calculation.
* Salt concentration is factored in to Tm Calculation.
* DNA length, MW, GC %, Concentration based on OD at 260 nm and Extinction coefficient are displayed.
Download (2.2MB)
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